The exact date of the Templars' arrival in Acqui is unknown, as the first mention of their presence dates back to 1186, when Pope Urban III ordered them and the Hospitallers to cease interfering in the parish affairs of the town, especially during pilgrimages and funerals. This only indicates that the Knights Templar, as well as the Knights Hospitaller, seem to have been well established in Acqui for many years and that they were very active there.

A document dated 1208 mentions that Brother Pietro, chaplain of Santa Margherita del Tempio in Acqui, declared before the provost of Acqui Cathedral that he owned land in the district of Acqui, in a place called Centenario, and that he had to pay a quarter of the income from this land to the cathedral.

Another document, also dated 1208 and issued in Turin, mentions Brother Oberto di Acqui. In 1220, yet another document tells us that the Order of the Temple owned property in Ponzone⁽¹⁾ and Morsasco⁽²⁾.

Finally, in 1239, a document signed by Pope Gregory IX tells us that the Templars of Genoa and Acqui had to pay part of the costs of a Genoese delegation's visit to Rome that same year.

In 1244, a provincial chapter was held in Piacenza (Plaisance). Among the Templars present was Brother Bonifacio de Ponzone, Commander of Acqui.

As far as Ponzone is concerned, we do not know whether the Templar estate was part of the House of Acqui or whether there was in fact an independent house there.

The name of Brother Marco, Commander of Acqui, is mentioned in a complaint filed by the cathedral chapter of Acqui against the House of the Temple for non-payment of rent for land occupied by the Order and belonging to the bishopric.

The last mention of the Templar house of Sainte Marguerite d'Acqui dates back to 1366, in documents related to the Order of Hospitallers.